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Structural and dynamic insights into redundant function of YTHDF proteins

Authors:
Y. Li; R.K. Bedi; E.V. Moroz-Omori; A. Caflisch

Journal: J. Chem. Inf. Model.
Year: 2020
Volume: 60
Issue: 12
Pages: 5932-5935
DOI: 10.1021/acs.jcim.0c01029
Type of Publication: Journal Article

Abstract:

Three YTH-domain family proteins (YTHDF1, YTHDF2, and YTHDF3) recognize the N-methyladenosine (mA) modification of mRNA in cells. However, the redundancy of their cellular functions has been disputed. We investigate their interactions with mA-containing RNA using X-ray crystallography and molecular dynamics (MD). The new X-ray structures and MD simulations show that the three proteins share identical interactions with the mA-containing RNA and have similar intrinsic plasticity, thus evidencing the redundant roles of the three proteins in cellular functions.